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Experimental study and significance of expanded human umbilical cord blood cells in vitro transplanted to BALB/C mice
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.0254-1785.2003.04.104
- Key Word:
- 脐带血;移植;小鼠
Abstract： 目的探讨经体外扩增的脐血造血细胞对小鼠造血重建功能的影响,寻求脐血用于成人移植的可行方法.方法将经致死剂量γ射线照射后的BALB/C小鼠,随机分为4组,每组10只.A组:每只移植2.5×106个新鲜脐血细胞;B组:每只分别移植1.25×106个新鲜脐血细胞和1.25×106个扩增培养1周的脐血细胞;C组:每只移植2.5×106个扩增培养1周的脐血细胞;D组: 每只仅输注0.5ml生理盐水.比较各组造血重建的差别.结果在脐血细胞移植后第13d, A组存活5只(50%),B组8只(80%),C组6只(60%), D组全部死亡.B组小鼠的死亡率最低.脐血细胞移植后14d,小鼠的WBC已开始回升,B组与C组的恢复明显较A组快,P<0.05.结论混合移植新鲜的和体外培养1周的脐血造血细胞,可能是脐血移植的较好办法.
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