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Regulation of early-effective cytokines on the expression of chemokine receptors and adhesion molecules in cord blood AC133+ cells
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.0254-1785.2003.04.102
- Key Word:
- 脐带血;受体,趋化因子;细胞粘附分子
Abstract： 目的探讨不同细胞因子组合对脐血AC133+细胞表面趋化因子受体(CXCR4)和粘附分子VLA4(CD49d)表达的影响.方法采用双色直接免疫荧光标记法,使用流式细胞仪测定新鲜分离的、不同培养条件下体外培养7d的脐血AC133+细胞表面CXCR4和VLA4(CD49d) 的表达情况.结果 26例新鲜脐血AC133+细胞中CXCR4的表达率为(46.3±11.5)% ; VLA4的表达率为(51.2±17.3)%.在无血清、有细胞因子存在的条件下,短期液体扩增培养后,脐血AC133+细胞中CXCR4和VLA4的表达率发生变化,其中在早期效应细胞因子SCF、FL和TPO组合下,CXCR4和VLA4表达率为(68.9±15.8)%和(73.6±19.8)%,与新鲜脐血比较,差异非常显著,P＜0.01.结论在体外短期扩增培养过程中,早期效应细胞因子能显著上调脐血AC133+细胞CXCR4和VLA4的表达;用SCF、FL和TPO组合扩增脐血,不仅能获得足够数量的造血干/祖细胞,还能增加造血干/祖细胞的迁移和归巢能力.
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