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Effect of Ly49A transfected lymphocytes on graft versus host disease and graft versus leukemia effect after allogeneic bone marrow transplantation
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.0254-1785.2003.01.103
- Key Word:
- 骨髓移植;杀伤细胞;受体,细胞表面;移植物抗宿主病
Abstract： 目的观察Ly49A基因转染淋巴细胞对异基因骨髓移植后移植物抗宿主病(GVHD)和移植物抗白血病效应(GVL)的影响.方法经逆转录病毒介导将Ly49A基因转染至C57BL/6小鼠的淋巴细胞,以C57BL/6(H-2b)小鼠为供者,以接种EL9611红白血病细胞的BALB/c(H-2d)小鼠为受者,进行脾淋巴细胞和骨髓细胞联合移植,建立异基因急性GVHD模型,观察Ly49A基因转染的淋巴细胞对GVHD和GVL的影响.结果在未进行移植的单纯照射组,小鼠的存活时间为(6.50±2.41)d;仅以环磷酰胺治疗的小鼠存活时间为(20.90±2.88)d;非转染淋巴细胞和骨髓细胞联合移植组的存活时间为(17.10±4.65)d;空载体转染淋巴细胞和骨髓细胞联合移植组的存活时间为(17.40±5.32)d;Ly49A转染淋巴细胞与骨髓细胞联合移植组的存活时间为(35.20±12.52)d,较上述各组明显延长(P=0.000).结论 Ly49A基因转染的淋巴细胞在异基因骨髓移植模型上具有一定程度的降低GVHD并保留GVL的作用.
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