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Experimental study of SDMC-2 solution for long-term hypothermic preservation of rat hearts
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.0254-1785.2002.06.105
- Key Word:
- 器官保存液;低温保藏;心脏移植;大鼠
Abstract： 目的开发一种应用简便、保存效果好的新心脏保存液.方法实验动物采用SD雄性大鼠.分为对照组(A组),Stanford液保存组(B组),SDMC-1液保存组(C组),SDMC-2液保存组(D组).实验组中,每组心脏均经某一种液体停搏、灌洗并保存8h,然后通过Langendorff灌流装置再灌注,分别测定保存末及再灌注后心肌的ATP、肌酸激酶(CK)、含水量及组织学检查,以及左心室功能的恢复、冠脉流量(CF).结果B、C、D三组各项检测指标与A组比较均有不同程度降低(P<0.05或0.01).左心室功能指标:D组优于B、C组;CF:D组高于B、C组;ATP、CK含量:保存末及再灌注后D组优于B、C组,再灌注前后比较,B、C组降低;B组水含量高且再灌注后加重;电镜:D组细胞结构损伤轻,B组损伤最重.结论SDMC-2液配方更为合理,长时间低温保存离体心脏,效果优良.可直接用于心脏停搏、原位灌洗、保存及移植过程中的灌注,简化了心脏保存过程.
- [1]Nyborg NC,Kevelaitis E,Menasche P.Coronary endothelial dysfunction of isolated hearts subjected to prolonged cold storage: patterns and contributing factors.[J].1999,18(3).
- [2]毕研文,宋惠民,李守先,等.自制SDMC-1液对热缺血冷保存狗心保护的实验研究[J].1997,(4).
- [3]H M, Piper,D, García-Dorado,M, Ovize.A fresh look at reperfusion injury.[J].1998,38.
- [4]Naya JL,Llosa JC,Rodriguez-Lambert JL,etc.Celsior, a novel cardioplegic solution for arrest and storage in heart transplantation.[J].2000,32(8).
- [5]Raymond Cartier,Charleen Hollmann,François Dagenais,etc.Effects of University of Wisconsin solution on endothelium-dependent coronary artery relaxation in the rat[J].1993,55(1).