You Position: Home > Paper
Expression of inducible-HSP70 in ischemia/reperfusion rat kidney
( views:174, downloads:0 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.0254-1785.2002.06.104
- Key Word:
- 缺血预处理;再灌注损伤;热休克蛋白质类70;肾脏
Abstract： 目的探讨肾脏组织在缺血-再灌注损伤过程中诱导型热休克蛋白70(Ihsp70)的基因表达.方法运用狭缝杂交印迹法,半定量测定Wistar大鼠肾脏在原位缺血-再灌注后皮层部和乳头部组织中Ihsp70Mrna的水平.结果在单纯缺血实验中,缺血10、30和60min三组,肾脏皮层部Ihsp70基因的表达与正常无缺血对照组差异无显著性;在缺血-再灌注实验中,肾脏皮层部和乳头部肾组织Ihsp70基因表达在缺血10min、再灌注2～72h各组与正常对照组比较,均未见明显的变化.而在缺血30和60min后再灌注2～72h各组中,4～48h各组均显著高于对照组;再灌注后72h与对照组差异无显著性.对肾脏皮层部和乳头部的峰值表达分析,乳头部大于皮层部.以再灌注时间为轴线的表达趋势可以看出,缺血30和60min的表达类似.结论在肾脏缺血再灌注过程中,Ihsp70基因呈现快速的一过性表达;一定程度的缺血损伤,是诱导肾脏在再灌注过程中Ihsp70基因表达的前提条件,但肾脏缺血损伤的程度并不影响其Ihsp70基因表达的强度;在缺血-再灌注损伤中,肾脏不同解剖部位诱导Ihsp70基因表达的强度不同,乳头部强于皮层部.
- [1]M I, Rauchman,J, Pullman,S R, Gullans.Induction of molecular chaperones by hyperosmotic stress in mouse inner medullary collecting duct cells.[J].1997,273(1 Pt 2).
- [2]T, Yoshioka,T, Bills,T, Moore-Jarrett,etc.Role of intrinsic antioxidant enzymes in renal oxidant injury.[J].1990,38.
- [3]Yamada K,Kawamura Y,Katano H,etc.Hypothermia modulates induction of hsp70 and c-jun mRNA in the rat brain after subarachnoid hemorrhage.[J].2000,17(3).
- [4]N, Honda,A, Hishida,K, Ikuma,etc.Acquired resistance to acute renal failure.[J].1987,31.
- [5]孙经建.热休克蛋白70的分布、调节及功能[J].1997,(1).
- [6]Fan C,Engelhardt JF,Zwacka RM.Therapeutic approaches for ischemia/reperfusion injury in the liver.[J].1999,77(8).
- [7]赵克森，金丽娟主编.病理生理学[M].1999.
- [8]胡晓燕,曲音波.热休克蛋白的研究进展[J].1999,(0S1).