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A large-scale scanning and comparative analysis of early response genes associated with dominant regulation
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.0254-1785.2002.02.103
- Key Word:
- 基因表达调控;白细胞介素10
Abstract： 目的动态观察白细胞介素10(IL-10)作用后,淋巴细胞早反应基因表达谱变化,为下一步评价患者免疫状态和诱导治疗提供理论依据.方法应用DNA微矩阵技术,分离正常人外周血单个核细胞(PBMC),予以抗CD3McAb及CD3McAb+IL-10刺激,抽提24 h PBMC mRNA,反转录后与芯片杂交,通过生物信息学方法比较分析.结果培养24h后,抗CD3McAb+IL-10组与抗CD3McAb组比较有15个基因发生改变,5个基因表达明显下调,10个基因上调,主要功能在于促进基因编码区连接,影响细胞骨架和运动,不同部位的磷酸化、去磷酸化,细胞识别,会促进细胞生长作用,细胞凋亡,信号传递的基因则重点涉及钙离子-钙调蛋白、G-蛋白信号通路.结论在IL-10作用下,一系列基因转录活化,其中许多先前与免疫细胞无明确功能的相关基因参与了T细胞活化与调控,是否该群基因即代表IL-10作用下淋巴细胞的基因表达标签,尚须进一步证实;要完全明确T细胞调控网络,还应全面分析T淋巴细胞基因表达谱,从中筛选差异有显著意义的基因,进行功能研究.
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