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Induction of donor-specific tolerance to skin g raft by intrathymic inoculation of recipient T cells trans-fected by re trovirus with donor MHC gene
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.0254-1785.2002.01.102
- Key Word:
- 主要组织相容性复合物;基因转移;胸腺;皮肤移植;免疫耐受
Abstract： 目的探讨受者胸腺内注射可表达供者主要组织相容性复合物(MHC)基因的受者T淋巴细胞诱导的免疫耐受的效果. 方法将携带供鼠(C57BL/6, H-2b)k位点基因cDNA的逆转录病毒载体质粒PXN(N2-B19-H-2Kb)经包装细胞PA317细胞包装为重组病毒后,感染体外培养的受鼠(Balb/c, H-2d)T淋巴细胞,再将此可表达供者MHC抗原的受者T淋巴细胞(H-2Kd b)回输受鼠胸腺内,然后将供鼠(H-2b)的皮肤移植给受鼠(H-2 d),观察免疫耐受的诱导情况. 结果外源性MHC基因(H-2Kb)整合到靶细胞(H-2d)染色体DNA,并有效地转录,在细胞膜上有H-2Kb分子表达;对照组移植皮肤平均存活时间(M ST)为9 d;注射单克隆抗体对照组MST为11 d;第三供者组MST为11.5 d;实验组( 胸腺回输H-2Kdb嵌合体T淋巴细胞)MST为35 d;实验组脾细胞对刀豆素A(ConA) 的增殖反应在正常范围,单向混合淋巴细胞反应对第三供者的脾细胞反应正常,但对特异供者的脾细胞无反应. 结论自身T淋巴细胞在胸腺内表达供者MHC抗原可在成年动物诱导出对供者移植物的特异性免疫耐受,无非特异性免疫抑制.
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