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Effect of varapamil and Na/K ion channel blockers on energy metabolism of human hepatocytes during low temperature preservation
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.0254-1785.2001.05.104
- Key Word:
- 肝;细胞;钙通道阻滞药;Na+-K+交换酶;能量代谢
Abstract： 目的研究维拉帕米及Na+-K+通道阻滞剂四乙基胺、奎尼丁和阿米洛利对低温保存成人肝细胞能量代谢的影响和对细胞的保护作用.方法用胶原酶消化法制备成人肝细胞,置于分别加入维拉帕米、四乙基胺+奎尼丁+阿米洛利、四乙基胺+奎尼丁+阿米洛利+维拉帕米的保存液中保存,高效液相色谱仪检测各组肝细胞中ATP、ADP及AMP的含量.结果在维拉帕米实验组,ATP水平在实验过程中始终高于低温保存对照组;前4个小时ADP平稳升高,以后逐渐减低;AMP呈缓慢升高趋势,至12*!h达峰值;总腺苷酸含量总体呈下降趋势,但平台期延长,下降缓慢;能量转换率的变化与总腺苷酸含量相似.在加用维拉帕米和Na+-K+通道阻滞剂的联合用药组,ATP缓慢降低到初期的50%以下;ADP初期缓慢上升,4*!h后逐渐下降;AMP呈缓慢上升趋势;总腺苷酸含量和能量转换率均较维拉帕米组高,提示细胞的活性保持良好.结论在低温保存成人肝细胞时加入维拉帕米和Na+-K+通道阻滞剂可降低肝细胞的能量消耗,它们对细胞的保护具有协同作用.
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