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Donor gene transfecting recipient to induce specific immunological tolerance

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
Issue:
2
DOI:
10.3760/cma.j.issn.0254-1785.2001.02.102
Key Word:
胸腺;大鼠;免疫耐受

Abstract: Objective To investigate the role of the donor specific MHC Class I RT1.Aa cDNA molecule in the induction of immunological tolerance and possible mechanism. Methods A rat model of cervical heterotopic cardiac transplantation was established. The donor MHC Class I RT1.Aa gene fragment was used to transfect recipient autologous myoblasts (MB) which were inoculated with recipient thymus. The survival time of the grafts was observed and the immunological tolerance evaluated. Results The autologous MB inoculated with the transfected donor gene via thymus in combination with CsA had a graft survival time of (96.13±12.91) days, significantly longer than that in the experiment groups (P<0.05). Cpm value of the mixed lymphocyte reaction (MLR) in the control group was higher than in the experimental group correspondingly. Conclusions Immunological tolerance could be induced by donor specific MHC class I RT1.Aa antigen to a donor-type cardiac allograft. In the immunological tolerance, the efficacy induced by RT1.Aa was better than by donor immune activated cells and the effects of central tolerance induced via the approach of thymus was superior to those via the peripheral way.

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