Abstract： This article reported a rapid salting out procedure for extracting DNA from human nucleated cells and clinical practice in donor-recipient matching by HLA genotyping.This method was compared to the standard phenol/chloroform extraction in 210 samples from different tissues.The results showed that the salting out method was a simple,rapid and nontoxic extraction procedure.The methodwas performed in an overall time of 110 minutes.The DNA quality obtained by this simple technique was comparable to that obtained by phenol-chloroform extraction.None of the 210 tests were failed.The DNAs obtained by both methods have been successfully used for donor-recipient genotyping.The two methods showed completely concordant results.It was demonstrated that the DNA extracted by the rapid salting out method was suitable for clinical DNA typing in tissue and organ transplantations.