Expression and significance of CD146 in peripheral blood of patients with Henoch-Sch(o)nlein purpura
- Author:
- No author available
- Journal Title:
- Chinese Journal of Dermatology
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.0412-4030.2012.04.019
- Key Word:
- No keyword available
Abstract： Objective To detect the expression of CD146 in sera and peripheral blood mononuclear cells (PBMCs)of patients with Henoch-Sch(o)nlein purpura (HSP),and to assess its role in the pathogenesis of HSP.Methods Sixty patients with HSP in acute or recovery stage and 20 normal human controls were included in this study.Enzyme-linked immunosorbent assay (ELISA)was performed to measure the expression levels of serum soluble CD146 (sCD146),and real-time fluorescence-based quantitative reverse transcription-PCR to detect the mRNA expression levels of CD146 in PBMCs.The correlation of the above parameters with disease activity was assessed.Results The levels of serum sCD146 and CD146 mRNA in PBMCs in patients with HSP in recovery stage were significantly higher than those in the normal human controls (0.52 ± 0.15) ng/L vs. (0.23 ± 0.08) ng/L,t =8.90,P ＜ 0.05; 14.18 ± 2.49 vs.10.08 ± 2.08,t =6.92,P ＜ 0.05),but lower than those in patients with HSP in acute stage (0.84 ± 0.49) ng/L,t =3.44,P ＜ 0.05; 26.34 ± 2.55,t =18.69,P ＜0.05).Conclusion CD146 may participate in the pathogenesis of HSP.
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