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Effects of epidermal proteins and lamellar bodies on epidermal barrier in glucocorticoid-dependent dermatitis

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Author:
No author available
Journal Title:
Chinese Journal of Dermatology
Issue:
2
DOI:
10.3760/cma.j.issn.0412-4030.2012.02.005
Key Word:
皮炎,激素依赖性;角蛋白;板层小体;皮肤;屏障;Dermatitis,glucocorticoid-dependent;Keratins;Lamellar bodies;Skin;Barrier

Abstract: Objective To explore the effects of epidermal proteins and lamellar bodies on skin barrier in glucocorticoid-dependent dermatitis.Methods Totally,60 patients with glucocorticoid-dependent dermatitis and 40 normal human controls were eligible for this study.A noninvasive method using TewameterTM was applied to determine transepidermal water loss (TEWL) value in these subjects.Tissue specimens were obtained from the lesions of 13 patients with glucocorticoid-dependent dermatitis and normal skin of 10 human controls.Subsequently,haematoxylin and eosin (HE) staining was performed to observe the histopathological changes,immunohistochemistry to detect the protein expressions of K6,K10,K14,K15,loricrin,filaggrin,involucrin in epidermis,and electron microscopy (EM) to estimate the density of lamellar bodies in tissue specimens.Results Compared with the normal controls,the patients displayed an elevated TEWL value (P < 0.05),which suggested an impaired epidermal barrier.Histopathology of lesions revealed nonspecific inflammatorychanges with marked differences between different clinical types of glucocorticoid-dependent dermatitis.Immunohistochemistry revealed an attenuated expression of K10,K14,loricrin,filaggrin,involucrin and abnormal expression of K15 in lesional epidermis compared with the normal epidermis (all P < 0.05),hinting a suppression of epidermal differentiation and proliferation as well as an impairment of cornified envelope structure.The number and density of lamellar bodies were also reduced in lesional epidermis compared with the control epidermis.Conclusions Compared with normal skin,the structure of skin barrier is impaired in lesions of glucocorticoid-dependent dermatitis,to restore skin barrier is essential for the treatment of this entity.

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