Primary exploration on identification of pathogenic Trichosporon spp. with rDNA-RFLP analysis
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 8
- DOI:
- 10.3760/cma.j.issn.0412-4030.2010.08.005
- Key Word:
- 毛孢子菌属;DNA,核糖体;多态性,限制性片段长度;Trichosporon;DNA,ribosomal;Polymorphism,restriction fragment length
Abstract： Objective To genotype Trichosporon spp. with rDNA-ITSAGSl-RFLP analysis followed by cluster analysis, and attempt to apply this method to rapid species identification of human pathogenic Trichosporon spp.. Methods Fourteen strains of Trichosporon, which belonged to 8 species, were collected. The rDNA-ITS/IGSl regions were amplified by PCR and sequenced. Simultaneously, the amplicons were digested separately with restriction enzymes, including Hae III, Hha I , Hae IH and Hha I , Hinf I , Msp I and Taq I . Results The 8 species of Trichosporon could be classified into 4 subgroups with rDNA-ITS-RFLP, while inter-species identification of all the 14 strains from 8 species of Trichosporon could be realized with rDNA-IGSl-RFLP. Also, those genotypes of T. asahii which had relative long phylogenic distance could even be discriminated with rDNA-IGSl-RFLP. Conclusion The rDNA-ITS/IGSl-RFLP analysis is expected to be used in rapid interspecific identification of genus Trichosporon.
- [1]夏志宽,杨蓉娅,王文岭,等.临床分离阿萨希毛孢子菌IGS1区克隆及RFLP分析[J].2008,(8).
- [2]Gunn SR,Reveles XT,Hamlington JD,etc.Use of DNA sequencing analysis to confirm fungemia due to Trichosporon dermatis in a pediatric patient.[J].2006,44(3).
- [3]Colombo Arnaldo L,Chagas-Neto Thomas C,Chaves Guilherme M.Update on the Genus Trichosporon[J].2008,166(3).
- [4]Sugita T,Can E,Yalman N,etc.Two possible cases of Trichosporon infections in bone-marrow-transplanted children: the first case of T. japonicum isolated from clinical specimens.[J].2008,61(2).
- [5]Sugita T,Shinoda T,Matsushima T,etc.Sequence analysis of the ribosomal DNA intergenic spacer 1 regions of trichosporon species.[J].2002,40(5).
- [6]Sugita T,Ikeda R,Shinoda T,etc.Identification of medically relevant Trichosporon species based on sequences of internal transcribed spacer regions and construction of a database for Trichosporon identification.[J].1999,37(6).