You Position: Home > Paper
Effect of monochromatic 308 nm excimer light on cell cycle, apoptosis of and KIT expression in human melanocytes
( views:241, downloads:0 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 3
- DOI:
- 10.3321/j.issn:0412-4030.2008.03.006
- Key Word:
- 准分子光;黑素细胞;细胞凋亡;细胞周期;原癌基因蛋白质c-kit
Abstract： 目的 探讨308 nm单频准分子光对体外培养的人黑素细胞凋亡、细胞周期和KIT蛋白表达的影响.方法 在流式细胞仪上采用Annexin V检测0,20,40,80,160,300 mJ/cm2剂量308 nm单频准分子光照射后24 h黑素细胞的凋亡;采用碘化丙啶检测80 mJ/cm.照射后24 h细胞周期的变化.实时荧光PCR和Werstern印迹分别检测0,20,40,80,160,300 mJ/cm2剂量照射后24 h黑素细胞c-kit mRNA和KIT蛋白的表达.结果 照射后24 h,在小于160 mJ/cm2剂量下,黑素细胞凋亡无明显增加,但300 mJ/cm2时的凋亡率与对照相比较明显增加(P＜0.05).80 mJ/cm2剂量照射后24 h,S期细胞数明显高于其对照,分别为29.06%±0.75%和22.64%4±0.07%;低剂量308 nm单频准分子光照射后24 h,c-kit mRNA表达呈先增加后减少的趋势,80 mJ/cm2时达高峰,KIT蛋白的表达与mRNA的表达趋势一致.结论 低剂量308 nm单频准分子光(80 mJ/cm2)促进黑素细胞增殖和KIT的表达.
- [1]郭静,项蕾红,郑志忠,等.单频准分子光治疗白癜风的临床研究[J].2006,(1).
- [2]Imokawa G,Kobayashi T,Tsukamoto K,etc.Mechanisms underlying the dysfunction of melanocytes in vitiligo epidermis: role of SCF/KIT protein interactions and the downstream effector, MITF-M.[J].2004,202(4).
- [3]N V, Botchkareva,M, Khlgatian,B J, Longley,etc.SCF/c-kit signaling is required for cyclic regeneration of the hair pigmentation unit.[J].2001,15(3).