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The mRNA and protein expression of inducible nitric oxide synthase in cutaneous malignant melanoma
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 4
- DOI:
- 10.3760/j.issn:0412-4030.2007.04.011
- Key Word:
- 黑色素瘤;一氧化氮合酶;信使RNA
Abstract： 目的 探讨皮肤恶性黑素瘤中诱导型一氧化氮合酶(iNOS)的表达及其临床意义.方法 免疫组化方法检测30例皮肤恶性黑素瘤患者肿瘤组织中iNOS蛋白的表达,逆转录聚合酶链反应(RTPCR)检测其中6例患者肿瘤组织和肿瘤邻近正常组织中iNOS mRNA的表达.结果 iNOS在肿瘤组织中无论蛋白或mRNA阳性表达率皆明显高于邻近正常组织(P＜0.05).其中iNOS蛋白在无转移的恶性黑素瘤中阳性表达率为69.2%,有转移的恶性黑素瘤中阳性表达率为100%.结论 iNOS表达异常在恶性黑素瘤发生、发展与转移过程中可能起重要作用.
- [1]陶娟,涂亚庭.恶性黑素瘤内皮型一氧化氮合酶和血管内皮生长因子的表达[J].2004,(10).
- [2]Yoshiyuki Ishii,Tsutomu Ogura,Masayuki Tatemichi,etc.Induction of matrix metalloproteinase gene transcription by nitric oxide and mechanisms of MMP-1 gene induction in human melanoma cell lines[J].2003,103(2).
- [3]T, Goto,K, Haruma,Y, Kitadai,etc.Enhanced expression of inducible nitric oxide synthase and nitrotyrosine in gastric mucosa of gastric cancer patients.[J].1999,5(6).
- [4]S, Ekmekcioglu,J, Ellerhorst,C M, Smid,etc.Inducible nitric oxide synthase and nitrotyrosine in human metastatic melanoma tumors correlate with poor survival.[J].2000,6(12).
- [5]T E, Konopka,J E, Barker,T L, Bamford,etc.Nitric oxide synthase II gene disruption: implications for tumor growth and vascular endothelial growth factor production.[J].2001,61(7).
- [6]Paglierani M,Borgognoni L,Santucci M,etc.Inducible nitric oxide synthase expression in benign and malignant cutaneous melanocytic lesions.[J].2001,194(2).
- [7]Matsui C,Kagoura M,Morohashi M,etc.Immunohistochemical study of inducible nitric oxide synthase in skin cancers.[J].2001,28(9).
- [8]M, Joshi,J, Strandhoy,W L, White.Nitric oxide synthase activity is up-regulated in melanoma cell lines: a potential mechanism for metastases formation.[J].1996,6(2).