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In vitro antiviral susceptibilities of HSV-2 clinical isolates to acyclovir and valaciclovir
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 3
- DOI:
- 10.3760/j.issn:0412-4030.2007.03.004
- Key Word:
- 疱疹病毒2型,人;微生物敏感性试验;阿昔洛韦;伐昔洛韦
Abstract： 目的 测定南京地区收集的15株单纯疱疹病毒2型(HSV-2)对阿昔洛韦和伐昔洛韦的敏感性,初步建立空斑法提取阿昔洛韦耐药株.方法 Vero细胞培养法分离扩增出临床毒株,直接免疫荧光法分型,并用MTT法测定15株2型毒株对阿昔洛韦、伐昔洛韦的敏感性.体外阿昔洛韦诱导,空斑纯化HSV耐药株.结果 15株临床株对阿昔洛韦和伐昔洛韦的半数病毒抑制质量浓度(IC50)分别为0.0215～0.2799μg/mL(平均0.0766μg/mL),0.0549～0.4575μg/mL(平均0.1605μg/mL).生殖器疱疹初发组与复发组、用药组与未用药组对2种药物的敏感性采用Wilcoxon秩和检验,P值均＞0.05,差异无统计学意义.从15株HSV-2临床株中未提取到耐药空斑;从HSV-1 Sam实验室标准株提取到一个耐药空斑,MTT法测定阿昔洛韦IC50为13.36μg/mL;从HSV-2 333标准株中提取到9个耐药空斑,扩增后MTT法测定其对阿昔洛韦的IC50为1.04～5.08 μg/mL.结论 目前在南京地区收集的HSV-2型毒株对阿昔洛韦和伐昔洛韦敏感性较高,尚未发现耐药株.体外药物诱导、空斑纯化能较快提取到HSV耐药株.
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