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The effects of melanoma culture supernatants on production of IL-12 and expression of CD14 and CD1a by human monocytesin vitro
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 12
- DOI:
- 10.3760/j.issn:0412-4030.2006.12.004
- Key Word:
- 单核细胞;白细胞介素12;抗原,CD14;抗原,CD1;M14黑素瘤细胞培养上清液
Abstract： 目的 探讨M14黑素瘤细胞培养上清液(MCS)在体外对人外周血单核细胞分泌白介素12(IL-12)以及单核细胞向树突细胞分化的影响.方法 分离并获得人外周血单核细胞,添加不同量的MCS与单核细胞共培养,用酶联免疫吸附法(ELISA)检测单核细胞IL-12的分泌情况;将MCS与单核细胞连续5 d共培养,用流式细胞仪分析单核细胞表面CD14、CD1a的表达情况.结果 MCS对单核细胞分泌活性的影响呈浓度依赖关系,随MCS添加量的逐渐增加(25～100 μL),单核细胞分泌IL-12的量则呈逐渐降低趋势(158.40±21.37 pg/mL～56.90±15.60 pg/mL,P＜0.05);同时MCS妨碍单核细胞由CD14表型(5.47%±1.00%～17.87%±2.40%,P＜0.01)向树突细胞CD1a表型(41.00%±2.12%～20.00%±4.24%,P＜0.01)转化;且MCS抑制单核细胞IL-12分泌和抑制单核细胞向树突细胞表型转化的作用均能被细胞外信号调节激酶(ERK)特异性抑制剂(PD98059)所逆转(分别为:172.63±8.88 pg/mL,P＜0.05;8.67%±0.47%,P＜0.05;36.67%±0.71%,P＜0.05).结论 MCS可能通过分泌ERK刺激因子对单核细胞分泌IL-12p40和单核细胞分化起到抑制作用.
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