You Position: Home > Paper
Calcitonin gene-related peptide regulates the expression of vascular endothelial growth factor in human HaCaT keratinocytes
( views:138, downloads:12 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 12
- DOI:
- 10.3760/j.issn:0412-4030.2006.12.003
- Key Word:
- 降钙素基因相关肽;血管内皮生长因子类;HaCaT细胞
Abstract： 目的 探讨降钙素基因相关肽(CGRP)对角质形成细胞表达和分泌血管内皮生长因子(VEGF)的调控.方法 用实时定量PCR(RT-PCR)方法检测CGRP、CGRP1型受体拮抗剂CGRP8-37、细胞外信号调节激酶ERK1/2特异性的抑制剂PD98059、p38MAPK特异性拮抗剂SB203580对HaCaT角质形成细胞表达VEGF mRNA水平的影响;用酶联免疫吸附方法检测HaCaT细胞分泌至培养液中VEGF蛋白的水平.结果 CGRP时间依赖性地促进HaCaT细胞表达VEGF mRNA和分泌VEGF蛋白;CGRP8-37和PD98059均可明显抑制CGRP刺激的HaCaT细胞表达和分泌VEGF,SB203580不能减弱CGRP刺激的VEGF表达和分泌.结论 CGRP可以上调HaCaT细胞表达和分泌VEGF,CGRP1型受体及其相关的ERK1/2信号通路参与其调控.
- [1]聂本勇,范青源,郑茂荣,等.降钙素基因相关肽对HaCaT细胞一氧化氮合酶表达的影响[J].2004,(9).
- [2]李春阳,王英杰,王克玉,等.银屑病患者的心理因素及外周血单胺类物质的改变[J].2003,(3).
- [3]J.-L. Connat,L. Thomassin,D. Vandroux,etc.Calcitonin gene-related peptide partly protects cultured smooth muscle cells from apoptosis induced by an oxidative stress via activation of ERK1/2 MAPK[J].2003,1643(1).
- [4]Brooks DP,Aiyar N,Spielman WS,etc.Activation of multiple mitogen-activated protein kinases by recombinant calcitonin gene-related peptide receptor.[J].2000,389(2).
- [5]M, Detmar,L F, Brown,K P, Claffey,etc.Overexpression of vascular permeability factor/vascular endothelial growth factor and its receptors in psoriasis.[J].1994,180.