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Detection of deep-seated pathogenic fungi by polymerase chain reaction
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 8
- DOI:
- 10.3760/j.issn:0412-4030.2006.08.003
- Key Word:
- 真菌病;念珠菌属;曲霉菌,烟;隐球菌,新型;聚合酶链反应
Abstract： 目的 探讨PCR在可疑深部真菌感染临床标本检测中的应用.方法 对60份临床标本分别进行传统真菌培养以及真菌通用引物PCR和三重PCR检测.结果 真菌培养阳性共19份,其中白念珠菌10份、烟曲霉2份、新生隐球菌2份、其他菌株5份(包括光滑念珠菌2份、热带念珠菌2份、克柔念珠菌1份);真菌通用PCR检测共有21份阳性,经三重PCR检测共有15份阳性,分别为白念珠菌10份、烟曲霉3份、新生隐球菌2份.PCR方法与传统培养方法相比,检出率差异无统计学意义.结论 PCR检测可疑深部真菌感染临床标本快速简便,稳定性好,敏感性和特异性均较理想,适合临床实验室常规使用.
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