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Effects of estradiol benzoate and UVA radiation on collagen synthesis in cultured human fibroblasts
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 7
- DOI:
- 10.3760/j.issn:0412-4030.2006.07.005
- Key Word:
- 雌二醇;紫外线;成纤维细胞;胶原Ⅰ型;胶原Ⅲ型
Abstract： 目的探讨苯甲酸雌二醇对体外培养的人成纤维细胞增殖、胶原合成及其对UVA抑制胶原合成的影响.方法在培养的人成纤维细胞中分别加入不同剂量的苯甲酸雌二醇,继续培养48 h,用MTT法测定细胞的增殖情况,同时应用RT-PCR方法检测经不同剂量苯甲酸雌二醇及10 J/cm2 UVA处理后人成纤维细胞Ⅰ、Ⅲ型前胶原mRNA的表达情况.结果MTT法检测结果显示,苯甲酸雌二醇对体外培养的成纤维细胞增殖无明显促进作用.RT-PCR结果提示,苯甲酸雌二醇处理组两型前胶原的表达水平明显上调(P＜0.05),而UVA照射后两型前胶原的表达显著下降,苯甲酸雌二醇可在一定水平对抗其作用(P＜0.05).结论苯甲酸雌二醇对体外培养的成纤维细胞Ⅰ、Ⅲ型前胶原的合成有一定的促进作用,并可对抗紫外线抑制胶原合成的作用.
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