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A comparative study on the difference in the sequence of ERG11 gene between the yeast and hyphal form of Candida albicans by PCR-LIS-SSCP
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 7
- DOI:
- 10.3760/j.issn:0412-4030.2006.07.003
- Key Word:
- 念珠菌,白色;菌丝相;酵母相;ERG11基因
Abstract： 目的通过对白念珠菌菌丝相与酵母相ERG11基因PCR-LIS-SSCP图谱的比较,探讨两相细胞在DNA水平的差异.方法分别抽提16株来自同一亲本且对氟康唑敏感性不同的白念珠菌菌丝相与酵母相基因组DNA,根据ERG11基因序列设计7对引物,对其进行分段PCR扩增,扩增产物经单链变性后,以非变性聚丙烯酰胺凝胶进行SSCP分析.结果16株白念珠菌菌丝相与酵母相均能扩增出目的片段,SSCP图谱显示两相细胞的ERG11基因序列存在多位点差异.结论白念珠菌菌丝相与酵母相ERG11基因存在着差异.
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