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Effect of siRNA targeting survivin gene on the apoptosis of malignant melanoma cell line A375
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 4
- DOI:
- 10.3760/j.issn:0412-4030.2006.04.003
- Key Word:
- 黑色素瘤;RNA,小分子干扰;生存素
Abstract： 目的探讨RNA干扰技术抑制恶性黑素瘤细胞株A375生存素基因表达后,对A375细胞凋亡的影响.方法构建针对凋亡抑制基因生存素的siRNA真核表达载体pU-生存素-siRNA,用电穿孔法转染A375细胞,采用蛋白质印迹技术检测生存素的表达,并用流式细胞仪检测细胞凋亡的变化.结果转染pU-生存素-siRNA后,生存素在A375细胞中的表达(0.24±0.02)较对照组(0.98±0.21)明显下降,试验组细胞的凋亡率(83%)较对照组(28%)明显增加.结论通过RNA干扰技术可抑制生存素的表达,诱导A375细胞的凋亡增加.
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