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A comparative study between human skin equivalent and normal human skin
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 2
- DOI:
- 10.3760/j.issn:0412-4030.2006.02.001
- Key Word:
- 角蛋白细胞;黑素细胞;皮肤类似物
Abstract： 目的用正常人的皮肤角质形成细胞和黑素细胞在体外重建皮肤,为将来用于临床、生物及药理学研究奠定基础.方法从正常儿童的包皮分离、培养角质形成细胞、黑素细胞和成纤维细胞.用成纤维细胞和Ⅰ型鼠尾胶原制备真皮类似物.将角质形成细胞和黑素细胞接种到真皮类似物表面进行培养,制备表皮类似物.将重建的皮肤类似物和正常人皮肤进行对比研究.结果皮肤类似物的组织形态包括基底层、基底上层、角质层与正常人皮肤相似.与基底膜形成有关的标志,在皮肤类似物和正常人皮肤中的表达是相似的.角蛋白10、泛角蛋白在皮肤类似物和正常人皮肤中的表达也是相似的.结论组织形态和所检测的各种标志的表达显示,该皮肤类似物与正常人皮肤相似.
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