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In vitro Effects of the Traditional Chinese Medicine, Berberine, Matrine and Baicalin, on the Prolifera tion and Lipid Synthesis of Immortalized Human Sebocyte SZ95
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 11
- DOI:
- 10.3760/j.issn:0412-4030.2005.11.002
- Key Word:
- 小檗碱;黄芩甙;苦参碱;皮脂腺;细胞,培养的
Abstract： 目的研究中药单体盐酸小檗碱、黄芩苷和苦参碱对永生化人皮脂腺细胞(SZ95)增殖及脂质合成的影响.方法倒置显微镜观察药物作用24 h后细胞形态变化,确定药物的毒性浓度;用四甲基偶氮唑盐(MTF)法检测不同浓度的药物在作用24、48及72 h对SZ95细胞增殖的影响;用流式细胞仪检测药物作用48 h后尼罗红染色的SZ95细胞内脂质含量的变化.结果毒性浓度黄芩苷为1×10-3mol/L,盐酸小檗碱为1×10-4 mol/L,苦参碱在1×10-3mol/L以下细胞未见形态变化.MTT法显示,盐酸小檗碱呈时间和剂量依赖性抑制SZ95细胞的增殖,IC50值48 h为2.9×10-5mol/L,72 h为1.4×10-5mol/L;黄芩苷在5×10-4mol/L以下、苦参碱在1×10-3mol/L以下对SZ95细胞增殖无影响.1×10-4mol/L黄芩苷作用48 h使细胞平均荧光强度下降30.1%(P＜0.05);1×10-6mol/L盐酸小檗碱下降7.9%,但差异无统计学意义(P＞0.05);1×10-3mol/L苦参碱则促进脂质合成(26.9%).结论盐酸小檗碱、黄芩苷等中药单体可能具有抑制皮脂腺细胞增殖或脂质合成的作用.
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