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Detection and Genotyping of Treponema pallidum by a Nested PCR
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 9
- DOI:
- 10.3760/j.issn:0412-4030.2005.09.005
- Key Word:
- 梅毒;密螺旋体,苍白;基因型
Abstract： 目的探讨广州地区梅毒螺旋体基因型分布,确定其分子流行病学特点.方法收集2002-2004年间连续就诊的疑似梅毒患者的生殖器溃疡标本,用暗视野显微镜和巢式PCR检测梅毒螺旋体,阳性者进行巢式PCR扩增酸性重复蛋白基因(arp)和苍白螺旋体重复基因族(tpr),凝胶电泳分析arp基因重复序列个数和tpr基因的Mse Ⅰ酶切片段多态性.根据Pillay标准基因分型.结果共检测62例疑似硬下疳病例,33例(53.2%)暗视野镜检发现梅毒螺旋体;54例PCR检测阳性,阳性率为87.1%.47例arp基因分型以14型为主(36例占76.6%),49例tpr基因分型以d型为主(39例占79.6%),47例双重基因分型发现7个基因型,依次为14 d 31例占66.0%,13 d 5例占10.6%,14 b 4例占8.5%,12 b 3例占6.4%,12 d 2例占4.3%,15 d和14 i各1例占2.2%.结论巢式PCR法检测梅毒螺旋体具有较高的敏感性,广州地区梅毒螺旋体基因型以14 d型为优势型.梅毒的早期诊断和基因分型对于梅毒的防治有重要意义.
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