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Effects of Recombinant Chimeric Toxin Dsg3EC1-2PE40 on T and B Lymphocytes from Pemphigus Vulgaris
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 9
- DOI:
- 10.3760/cma.j.issn.0412-4030.2005.09.002
- Key Word:
- 天疱疮;免疫毒素类;桥粒芯糖蛋白
Abstract： 目的探讨重组嵌合毒素Dsg3EC1-2PE40对寻常型天疱疮患者外周血淋巴细胞的影响.方法对重组嵌合毒素Dsg3EC1-2PE40进行表达,并分离纯化;分别以不同浓度重组嵌合毒素作用于寻常型天疱疮患者外周血淋巴细胞,通过ELISPOT法、MTT比色试验及3H-TdR掺入试验来观察重组嵌合毒素对T、B淋巴细胞的影响.结果纯化后的重组嵌合毒素纯度达80%以上,它能够特异性与天疱疮抗体IgG结合,而与正常人IgG不结合.重组嵌合毒素可使寻常型天疱疮外周血产生抗体的B细胞数量减少约40%左右;在重组嵌合毒素作用下,T细胞的增殖、代谢较重组Dsg3EC1-2明显降低,两者间差异有统计学意义(P<0.01).结论重组嵌合毒素Dsg3EC1-2PE40可以抑制寻常型天疱疮外周血B细胞的抗体产生作用,对寻常型天疱疮外周血T细胞具有明显的抑制或杀伤作用.
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