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Development of PCR-RFLP for Identification of Eight Mycobacterial Species
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 9
- DOI:
- 10.3760/j.issn:0412-4030.2005.09.001
- Key Word:
- 分枝杆菌,结核;分枝杆菌,鸟;分枝杆菌,非典型性
Abstract： 目的建立检测8种常见皮肤分枝杆菌的PCR-RFLP方法.方法①用通用引物对分枝杆菌的热休克蛋白hsp65基因序列进行PCR扩增;②用限制性内切酶BstEⅡ和HaeⅢ对扩增产物进行消化,根据所得片段确定各分枝杆菌特征性的酶切图谱,从而对分枝杆菌进行鉴别.结果 8种常见皮肤分枝杆菌感染标准株(鸟、胞内、堪萨斯、结核、瘰疬、海、偶发、龟分枝杆菌)的酶切图谱与文献报道一致.结论 PCR-RFLP方法可用以检测鉴定皮肤分枝杆菌.
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