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Characterization of Pseudallescheria boydii and Scedosporium apiospermum by Random Amplification of Polymorphic DNA Assay
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 8
- DOI:
- 10.3760/j.issn:0412-4030.2005.08.006
- Key Word:
- 假霉样真菌属;丝孢菌属;随机扩增多态DNA技术
Abstract： 目的了解波氏假阿利什菌和尖端赛多孢子菌的基因学特征,研究DNA分型与菌种来源的关系.方法采用随机扩增多态性DNA分析(RAPD)方法.结果3种引物可将来自5个国家的13株波氏假阿利什菌和18株尖端赛多孢子菌分为31个基因型.多引物聚类分析所得树状图显示,除来自哥伦比亚土壤的3株波氏假阿利什菌外,其他受试菌株无地域性群集分布特点.但受试菌株中的多数波氏假阿利什菌和尖端赛多孢子菌株分别聚集成一群.结论波氏假阿利什菌和尖端赛多孢子菌存在较大株间差异,致病菌没有明显的地域性分布趋势,RAPD分型聚类分析结果与形态学分类之间具有一定一致性.
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