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Preparation and Characterization of Hybridoma Strains Secreting Natural IgM Against Keratin
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 4
- DOI:
- 10.3760/j.issn:0412-4030.2005.04.004
- Key Word:
- 抗体,单克隆;免疫球蛋白M;杂交瘤;角蛋白
Abstract： 目的制备天然属性的抗角蛋白单克隆IgM抗体.方法取饲养在无特殊病原体条件下BALB/c小鼠的脾细胞,直接与SP2/0细胞融合.以提取的角蛋白抗原对阳性杂交瘤细胞生长孔进行ELISA筛选.采用免疫组化、免疫印迹技术对获得的抗体进行鉴定.结果不经任何免疫,细胞融合率为60%;14%的杂交瘤细胞生长孔上清液与角蛋白抗原结合;获得3株稳定分泌IgM型天然抗角蛋白单克隆抗体的杂交瘤细胞株.免疫组化结果初步显示天然抗角蛋白IgM可以和表皮、皮脂腺、毛囊、肌肉组织结合.结论正常小鼠脾脏内存在自发分泌天然抗角蛋白IgM的B淋巴细胞.
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