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The Effect of Candida albicans on the Expression Level of Human β-defensin-2 mRNA in Keratinocytes in vitro
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 4
- DOI:
- 10.3760/j.issn:0412-4030.2005.04.002
- Key Word:
- 念珠菌,白色;β防御素;角蛋白细胞;甘露聚糖类
Abstract： 目的探讨白念珠菌及其菌体成分对人角质形成细胞β防御素-2表达的影响.方法采用酶解、反复冻融、超声破碎和超速离心等方法制备真菌成分;用逆转录聚合酶链反应(RT-PCR)方法检测白念珠菌及其菌体成分刺激人角质形成细胞24 h后β防御素-2 mRNA表达.结果正常培养的人角质形成细胞和角质形成细胞永生细胞系HaCaT均有β防御素-2 mRNA的少量表达,白念珠菌、白念珠菌细胞壁提取物和纯甘露聚糖可以使β防御素-2 mRNA表达增强(P值分别＜0.01、0.05和0.01),而白念珠菌培养上清液和细胞浆提取物对β防御素-2表达无明显影响(P＞0.05).结论白念珠菌可上调角质形成细胞β防御素-2 mRNA的表达,甘露聚糖可能是起诱导作用的活性成分.角质形成细胞通过表达β防御素-2在皮肤黏膜的抗感染防御机制中发挥重要作用.
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