You Position: Home > Paper
HPV Infection and its Correlation with MMP-9 and TIMP-1 Expression in Vulvar Squamous Cell Carcinoma
( views:325, downloads:0 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 12
- DOI:
- 10.3760/j.issn:0412-4030.2004.12.005
- Key Word:
- 癌,鳞状细胞;乳头状瘤病毒,人;明胶酶B;金属蛋白酶1组织抑制剂
Abstract： 目的探讨人乳头状瘤病毒(HPV)6/11、16/18在外阴鳞状细胞癌(简称外阴鳞癌)组织中的感染情况及与基质金属蛋白酶9(MMP-9)和金属蛋白酶抑制剂1(TIMP-1)蛋白表达的关系.方法用原位杂交法检测HPV6/11、16/18在26例外阴鳞癌、21例外阴上皮内瘤病变(VIN)及10例外阴正常皮肤组织中的感染情况.同时用免疫组化法检测MMP-9、TIMP-1蛋白的表达.结果HPV6/11、16/18在外阴鳞癌、VIN中的感染率分别为69.23%(18/26)和38.46%(10/26),42.86%(9/21)和28.57%(6/21),正常对照组没有感染.MMP-9、TIMP-1蛋白在外阴鳞癌、VIN、正常对照组中的阳性表达率分别为92.31%(24/26)和76.92%(20/26),90.48%(19/21)和80.95%(17/21),80.00%(8/10)和50.00%(5/10).HPV6/11的外阴鳞癌组、VIN组与正常对照组比较差异有显著性(P《0.05),HPV16/18的外阴鳞癌组与正常对照组比较差异有显著性.外阴病变各组MMP-9、TIMP-1阳性表达率与正常对照组比较差异均有显著性.在外阴鳞癌中MMP-9蛋白表达较TIMP-1蛋白表达强(P《0.05).MMP-9在有HPV6/11和HPV16/18感染患者中的表达较无感染者强,而TIMP-1蛋白的表达差异无显著性.结论在外阴鳞癌的发生发展中HPV的感染起一定作用,当MMP-9的表达强于TIMP-1表达时可能是外阴鳞癌侵袭的一个指标,HPV感染可能会进一步引起外阴鳞癌的浸润和转移.
- [1]樊翌明,李慧忠,吴志华,等.基质金属蛋白酶10、金属蛋白酶组织抑制因子2等在皮肤鳞状细胞癌中的表达[J].2002,(5).
- [2]L A, Brinton,P C, Nasca,K, Mallin,etc.Case-control study of cancer of the vulva.[J].1990,75.
- [3]Zeigler ME,Johnson TM,Schmidt T,etc.Collagenolytic and gelatinolytic matrix metalloproteinases and their inhibitors in basal cell carcinoma of skin: comparison with normal skin.[J].2000,82(3).
- [4]K, Tamakoshi,F, Kikkawa,A, Nawa,etc.Characterization of extracellular matrix-degrading proteinase and its inhibitor in gynecologic cancer tissues with clinically different metastatic form.[J].1995,76(12).
- [5]G. Torre,R. Donghi,A. Longoni,etc.HPV DNA in Intraepithelial Neoplasia and Carcinoma of the Vulva and Penis[J].1992,1(1).
- [6]H.Y.S Ngan,A.N.Y Cheung,S.S Liu,etc.Abnormal expression or mutation of TP53 and HPV in vulvar cancer[J].1999,35(3).
- [7]M J, Kagie,G G, Kenter,R A, Tollenaar,etc.P53 Protein Overexpression is Common and Independent of Human Papillomavirus Infection in Squamous Cell Carcinoma of the Vulva.[J].1997,80(7).