You Position: Home > Paper
Preliminary Study on Molecular Subtyping of Treponema pallidum in Hengyang and Jiangmen Regions
( views:220, downloads:12 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 12
- DOI:
- 10.3760/j.issn:0412-4030.2004.12.003
- Key Word:
- 密螺旋体,苍白;分子亚型
Abstract： 目的了解湖南衡阳和广东江门地区梅毒螺旋体(TP)基因亚型的分布.方法收集衡阳和江门地区疑似硬下疳病例的标本85份,经TP polA PCR筛选后,阳性者用PCR扩增各标本中TP的arp和tpr基因,酶切tpr基因扩增产物,分析各TP菌株arp基因长度和tpr基因的限制性片段长度多态性(RFLP),进行分型.结果TP polA PCR阳性者69例,可用于分型者57例,分为10个亚型,其中14d亚型为26例,其他亚型包括10d(1),12a(3),12g(2),13d(6),14a(5),14b(2),14f(6),15d(5),16d(1);衡阳和江门地区TP基因亚型分布大致相同.结论衡阳和江门地区存在TP的多亚型,都以14d亚型为主(占45.6%),无明显地区性差异.
- [1]Ballard RC,Pillay A,Liu H,etc.Molecular typing of Treponema pallidum in South Africa: cross-sectional studies.[J].2002,40(1).
- [2]Morse SA,Pillay A,Liu H,etc.Molecular subtyping of Treponema pallidum subspecies pallidum.[J].1998,25(8).
- [3]Rodes B,Liu H,Chen CY,etc.New tests for syphilis: rational design of a PCR method for detection of Treponema pallidum in clinical specimens using unique regions of the DNA polymerase I gene.[J].2001,39(5).
- [4]Steiner B,Mickey T,Pillay A,etc.Molecular subtyping of Treponema pallidum in an Arizona County with increasing syphilis morbidity: use of specimens from ulcers and blood.[J].2001,183(11).