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Study on the In Vitro Model for Th1 Type Shifting
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 12
- DOI:
- 10.3760/j.issn:0412-4030.2004.12.002
- Key Word:
- 白细胞介素12;白细胞介素4;干扰素Ⅱ型;脂多糖类;伴刀豆球蛋白A
Abstract： 目的建立小鼠脾细胞体外生成Th1型细胞因子的反应模式,以适应研究免疫治疗药物作用机制的要求.方法ELISA检测单核/巨噬细胞系统活化剂脂多糖(LPS)或T细胞丝裂原伴刀豆球蛋白A(ConA)在不同剂量或两种诱导剂不同剂量组合的条件下,诱导BALB/c小鼠脾细胞分泌的白介素12(IL-12)、干扰素γ(IFN-γ)和白介素4(IL-4)的产量.结果LPS能诱导BALB/c小鼠脾细胞分泌IL-12.ConA诱导BALB/c小鼠脾细胞分泌IFN-γ的最低质量浓度是0.25 mg/mL.不同浓度LPS与0.25mg/mLConA联合诱导BALB/c小鼠脾细胞分泌IFN-γ时,LPS表现出协同效应,IL-12与IFN-γ呈正相关,IL-4与IFN-γ呈负相关.结论LPS和ConA体外联合可诱导鼠脾细胞向Th1型细胞反应方向发展.
- [1]F S, Sutterwala,G J, Noel,R, Clynes,etc.Selective suppression of interleukin-12 induction after macrophage receptor ligation.[J].1997,185.
- [2]Verhasselt V,Willems F,Haeffner Cavaillon N,etc.Bacterial lipopolysaccharide stimulates the production of cytokines and the expression of costimulatory molecules by human peripheral blood dendritic cells: evidence for a soluble CD14-dependent pathway.[J].1997,158(6).
- [3]J, Le,J X, Lin,D, Henriksen-DeStefano,etc.Bacterial lipopolysaccharide-induced interferon-gamma production: roles of interleukin 1 and interleukin 2.[J].1986,136.
- [4]Orfanos CE,Goerdt S.Other functions, other genes: alternative activation of antigen-presenting cells.[J].1999,10(2).