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Studies on Susceptibility of Nine Antifungal Agents Against Dematiaceous Fungi by NCCLS M38-P Method
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 4
- DOI:
- 10.3760/j.issn:0412-4030.2004.04.003
- Key Word:
- 有丝分裂孢子真菌;抗生素类,抗真菌;微生物敏感性试验
Abstract： 目的检测暗色真菌对9种常用抗真菌药物的敏感性,评价美国国家实验室标准委员会(NCCLS)推荐的产孢丝状真菌药敏试验方案(M38-P)应用于暗色真菌药敏试验的可行性.方法参照M38-P方案,受试菌株共45株,包括卡氏枝孢14株,裴氏着色霉8株,紧密着色霉3株,疣状瓶霉16株,离蠕孢2株,德氏霉1株及弯孢1株.所用药物为氟康唑、氟胞嘧啶、伊曲康唑、咪康唑、益康唑、克霉唑、制霉菌素、两性霉素B和特比萘芬.菌悬液终浓度为(0.4～5)×106 cfu/mL,孵育温度35℃,培养时间5～7 d.结果45株暗色真菌对伊曲康唑和特比萘芬的敏感性较高,部分菌株对氟康唑、氟胞嘧啶、伊曲康唑、两性霉素B存在耐药现象.结论M38-P方案可用于暗色真菌的药敏试验研究,耐药菌株的发现提示在今后的治疗中药敏测试是必要的.
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