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Study on Microsatellite DNA Loss of Heterozygosity in p16 Gene of Psoriatic Kerafinocytes
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 3
- DOI:
- 10.3760/j.issn:0412-4030.2004.03.004
- Key Word:
- 银屑病;基因,p16;微卫星重复;杂合子丢失
Abstract： 目的探讨银屑病皮损区角质形成细胞p16基因D9S171,D9S1604微卫星位点杂合性缺失(loss of heterozygosity,LOH)与银屑病发病的关系.方法采用聚合酶链反应-变性聚丙烯酰胺凝胶电泳-银染技术对体外原代培养的银屑病皮损区和非皮损区角质形成细胞LOH进行分析.结果23例银屑病患者皮损区体外培养的角质形成细胞在D9S171和D9S1604两个微卫星位点,LOH分别为5例和10例.在非皮损区的相应位点LOH分别为2例和3例.D9S1604 LOH频率明显高于非皮损区角质形成细胞(P＜0.05);寻常性银屑病患者皮损区角质形成细胞中两位点LOH发生频率分别为23.5%(4/17)和47.1%(8/17),非寻常性银屑病分别为16.7%(1/6)和33.3%(2/6),两型银屑病两位点LOH发生频率相比差异均无显著性(P＞0.05).结论D9S1604微卫星位点LOH与银屑病的发生发展相关,而与临床类型相关无显著性.
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