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A Preliminary Study on the Antibody Secreted in Castleman's Disease Associated with Paraneoplastic Pemphigus
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 2
- DOI:
- 10.3760/j.issn:0412-4030.2004.02.004
- Key Word:
- 副肿瘤性天疱疮;序列分析
Abstract： 目的Castleman肿瘤组织细胞培养及培养细胞上清液中抗体成分性质的鉴定.方法分离副肿瘤性天疱疮患者的肿瘤淋巴细胞进行培养,将培养细胞上清液超滤离心获得浓缩的IgG抗体为一抗,分别以鼠膀胱冰冻切片为底物行间接免疫荧光检查,以正常皮肤蛋白提取物为底物进行免疫印迹试验.RT-PCR,克降测序分析培养肿瘤B细胞免疫球蛋白重链基因并与其他6例该病患者的基因相比较.结果培养细胞上清液中的IgG抗体能够在鼠膀胱上皮细胞间沉积;并且这些抗体能够识别皮肤中分子量分别为21 0 000和1 90 000的两种抗原成分.扩增培养肿瘤B细胞免疫球蛋白重链基因得到单一锐利条带,克隆测序发现该基因与另外6例患者的基因序列具有高度一致性.结论患者肿瘤组织能够分泌抗体,并且分泌抗体的性质与血液中抗体性质基本相同.
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