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Cloning of 119-264 Amino Acid Fragment of Ro52 Antigen and Analysis of Its Antigenicity
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 1
- DOI:
- 10.3760/j.issn:0412-4030.2004.01.006
- Key Word:
- 表位;DNA,重组;Ro52自身抗原
Abstract： 目的研究Ro52抗原的第119至第264位氨基酸片段(含亮氨酸拉链基序)在抗原表位构成中的作用.方法用PCR法从人类心脏cDNA第一链中扩增编码第¨9至第264位氨基酸的基因片段,定向插入表达质粒pMTY4,转导入宿主菌pop2136诱导表达融合蛋白,重组融合Ro52抗原片段纯化后用免疫印迹法进行抗原性检测.结果重组Ro52抗原的第119至第264位氨基酸片段的融合蛋白相对分子质量为28000,该融合蛋白在宿主菌中高水平表达,形成包涵体.在42份抗Ro52抗体阳性血清中,26份(61.90%)可与重组融合蛋白反应.结论所克隆的含亮氨酸拉链基序的第119至第264位氨基酸片段是Ro52抗原的重要抗原表位区段.
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