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Nucleosome Induced Systemic Lupus Erythematosus-like Disease in BALB/c Mice
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 1
- DOI:
- 10.3760/j.issn:0412-4030.2004.01.005
- Key Word:
- 核小体;红斑狼疮,系统性;自身抗体
Abstract： 目的了解核小体在系统性红斑狼疮(SLE)发病中的作用.方法用蔗糖连续密度梯度离心法从新鲜鸡血红细胞中提取核小体,皮下免疫BALB/c小鼠,采集小鼠血清和肾脏标本.ELISA方法检测其IgG型dsDNA抗体和ANA抗体水平;直接免疫荧光检测小鼠肾脏lgG型免疫复合物的沉积情况;组织病理观察小鼠肾脏损伤情况.结果核小体免疫后14 d,BALB/c小鼠血清中开始出现IgG型dsDNA抗体和ANA抗体,其滴度随时间延长逐渐升高;核小体免疫后35 d,小鼠肾脏出现大量IgG型免疫复合物沉积,光镜观察显示有明显肾脏损伤.对照组小鼠无明显自身抗体产生和肾脏病变.结论核小体可以诱导正常小鼠产生SLE样征候群,提示核小体参与SLE的发病.
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