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Expression and Mutation Analysis of the ING1 Tumor Suppressor in Human Basal Cell Epithelioma
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 11
- DOI:
- 10.3760/j.issn:0412-4030.2003.11.002
- Key Word:
- 癌,基底细胞;基因,肿瘤抑制;多态现象,单链构象;序列分析,DNA
Abstract： 目的研究肿瘤生长抑制基因ING1在基底细胞上皮瘤中是否存在过度表达及基因突变.方法搜集了54份基底细胞上皮瘤标本,运用免疫组织化学染色,DNA单链构象多态性分析(SSCP)及DNA序列测定的方法研究ING1基因在基底细胞上皮瘤中的表达及突变情况.结果免疫组化显示ING1基因在25%(6/24)基底细胞上皮瘤标本中过度表达.SSCP及DNA序列测定结果显示54例基底细胞上皮瘤标本中仅1例标本(1.8%)发生实质突变.突变发生在ING1基因编码区exon 2,因而可能影响所有ING1异构体结构,并影响PHD锌指基序的功能.结论ING1基因在基底细胞上皮瘤中无明显过度表达及突变.
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