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Establishment and Antibody Verification of the Animal Model of Sclerotic Skin Induced by Bleomycin
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 9
- DOI:
- 10.3760/j.issn:0412-4030.2003.09.004
- Key Word:
- 硬皮病,系统性;模型,动物;博来霉素;抗体,抗核
Abstract： 目的以博来霉素(Blm)诱导建立硬皮病小鼠模型并加以验证.方法将200μg/mLBlm 100μL注射在C3H/He小鼠(C3H小鼠)背部皮下,每日1次,连续3周,建立硬皮病小鼠模型,并经皮肤、肺组织病理学及血清自身抗体检测加以验证.结果注射200μg/mL Blm 3周的C3H小鼠,与对照组相比,注射区皮肤厚度明显增加(P＜0.001),胶原纤维组织化学指数显著升高(P＜0.0001),真皮出现均匀硬化表现;该小鼠肺泡间隔增厚,伴单一核细胞浸润,并可见肺组织小血管壁增厚;血清ANA阳性,间接免疫荧光法检测见细胞核呈粗斑点或细斑点状黄绿色荧光,有的斑点相互聚集成若干大斑点位于核中央,结合抗ENA抗体测定提示存在抗Scl-70抗体、抗着丝点抗体和抗U1-RNP抗体等.结论对C3H小鼠背部每日皮下注射200μg/mL Blm共3周,成功建立了硬皮病小鼠模型.
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