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Rapid Detection and Identification of Pathogenic Fungi of Some Deep Fungal Infections by PCR in Combination with Genescan Analysis
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 8
- DOI:
- 10.3760/j.issn:0412-4030.2003.08.005
- Key Word:
- 真菌病;序列分析,蛋白质;酵母菌;曲霉菌属;瓶霉菌属;孢子丝菌属;分支孢子菌属;毛霉菌属;镰刀菌属
Abstract： 目的用PCR法快速鉴定22种(23株)深部真菌病致病菌种.方法应用荧光标记的真菌通用引物ITS4与ITS86对22种(23株)深部真菌病致病菌种的菌悬液进行PCR扩增,扩增产物经ABIPRISMTM 377测序仪及基因扫描分析软件精确测定片段大小,与对照组--相应菌种采用传统方法抽提DNA后扩增片段的扫描分析结果相对照.结果①17种致病菌种菌悬液经ITS4、ITS86扩增出单一的片段(除了构巢曲霉和黑曲霉、白念珠菌和类星形念珠菌、裴氏着色真菌和皮炎外瓶霉片段长度相同).②菌悬液扩增片段的扫描分析结果与其DNA扩增结果相近,差异无显著性.③整个检定过程仅需6 h.结论采用ITS通用引物及菌悬液PCR检测法,结合基因扫描分析技术可准确、特异、快速、敏感地检测并鉴定出22种深部真菌病致病菌种,这将有望成为快速诊断深部真菌病的一种方法.
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