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Presentation of an Endogenous MHC Class Ⅰ Restricted Autoantigen by Keratinocytes in Vitro
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DERMATOLOGY
- Issue:
- 6
- DOI:
- 10.3760/j.issn:0412-4030.2003.06.005
- Key Word:
- 角蛋白细胞;抗原提呈;CD8阳性T淋巴细胞
Abstract： 目的探讨角质形成细胞对抗原受体特异的CD8阳性T细胞的抗原呈递作用.方法利用已建立的转染细胞系PAM-OK(可表达H-2Kb/卵白蛋白多肽复合体,氨基酸257-264)及对照细胞系,刺激幼稚或已被预激活的卵白蛋白特异的CD8阳性T细胞(H-2Kb)作为反应细胞,以3H-胸腺嘧啶掺入法检测T细胞的增殖反应.结果经γ干扰素刺激表达足够的抗原后,PAM-OK可刺激预激活的特异T细胞发生增殖反应,而不能刺激幼稚的T细胞发生反应.结论角质形成细胞有使预激活的抗原特异的T细胞发生增殖的潜能,该结果有助于解释某些皮肤病局部免疫反应持续存在的现象.
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