Abstract： Objective To evaluate the diagnostic and therapeutic values of two non invasive test methods for Neisseria gonohoeae. Methods The swab culture, urinary sediment culture and “ gold standard method” － swab culture were used to detect gonocococal strains. The results were analyzed to compare the validity, sensitivity and specificity of these three methods. Results There is no significant difference in the validity, sensitivity and specificity among the three methods (Male： 94.80％ 、 94.81％ and 95.35％ ； Female： 94.35％ 、 96.97％ and 84.10％ , P >0.05, respectively). Conclusions Non invasive urinary sediment culture and PCR－ hybridocomb test may be suitable to replace the swab culture method in STD clinic, and the urinary sediment culture is highly recommended.