Abstract： Objective To observe the acute and long-term toxicity of niuhuang jiedu dripping pill.Methods Maximum tolerence dose was determined on mice.Long-term(90 days) toxicity test was studied on rats.Results LD50 of niuhuang jiedu dripping pill given orally was more than 9 g/(kg·d),and there was no obvious long-term toxicity in general conditions,peripheral blood picture,function of liver and kidney and pathological histology in rats.Conclusion Niuhuang jiedu dripping pill is safe.