Abstract: Objective To study the cytotoxic T lymphocyte cells(CTLs)induced by tumor soluble antigen (TSA)and super-antigen staphylococcal enterotoxin C(SEC)and its anti-tumor effect in vitro.Methods The experiment was divided into control group(lymphocyte cells)and experiment group(SEC + TSA + lymphocyte cells).Lymphocyte cells were isolated from peripheral blood of cancer patients and then were induced by TSA and SEC in vitro, the levels of proliferation, cell phenotype and killing activity of CTLs were tested. Results Proliferation activity of lymphocyte cells group stimulated by TSA and SEC was the strongest.CD3 was expressed in control group and experiment group,and the CDs+ expressed in experiment group was higher than that in control group(49.07% vs 27.52%,P <0.05).When the ratio of effect and target was 20:1,the kill rates of CTLs on CALU-6,the lung cancer cells and Hela cells were higher than those when the ration was 10:1,and the kill rates of CTLs on CALU-6,the lung cancer cells were higher than that of Hela cells(P < 0.05).Conclusion The CTLs modified by super-antigen SEC and tumor antigen can induce high anti-tumor efficiency.