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Determination of vandetanib in rat plasma by high performance liquid chromatography

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Author:
CHEN Ting-xia(Department of Pharmacy, East Hospital , First people's of Lianyungang City, Jiangsu Province, Lianyungang 222042)
Journal Title:
China Medicine
Issue:
Volume 07, Issue 06, 2012
DOI:
10.3760/cma.j.issn.1673-4777.2012.06.036
Key Word:
Vandetanib;Chromatography, high pressure liquid;Rat

Abstract: Objective To develop a high performance liquid chromatography(HPLC)method for the determination of vandetanib in rat plasma.Methods The plasma samples were extracted and separated by an Agilent HC-C18 column,using methanol-0.02 mol/L sodium dihydrogen phosphate (80∶20 )as the mobile phase.The flow rate was 1.0 ml/min.The fractions were simultaneously detected by DAD with wavelength at 270 nm.Results The method was linear for vandetanib in the range of 0.5-10 mg/L.The correlation coefficient was 0.997 and the lower limit of quantification was 0.5 mg/L.The matrix effect was from 95.2% to 103.7%.The extraction recovery was in the range of 73.1%-87.9%.Conclusions The HPLC method is simple,sensitive and accurate.It is suitable for the determination of vandetanib in rat plasma.

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