Human gene p93 transgenic mouse model and its function exploration
- Author:
- No author available
- Journal Title:
- China Medicine
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1673-4777.2012.01.001
- Key Word:
- p93基因;转基因鼠;构建;心肌酶;血清水平;p93 gene;Transgenic mice;Construct;Myocardial zymogram;Serum level
Abstract： Objective To explore the role of p93 gene in heart development and heart diseases.Methods The recombined α-myosin heary chain ( α-MHC-p93 ) vector was constructed and a model of Founder transgenic mice was established by microinjection.The integration and heredity of the foreign gene were tested by poly meras chain reaction(PCR) and Southern blotting.The phenotype and the serum level were performed to observe the function of p93 gene in transgenic mice.Results The plasmid vector namelyα-MHC-p93 was constructed successfully.PCR and Southern blotting results showed that 3 of the F1 and 1 of F2 transgenic mice were positive with p93 gene.Observing the phenotype of p93 transgenic mouse model did not detect the atrioventricular septal defect (AVSD).The myocardial zymogram of p93 transgenic mice serum was abnormal,especially Ca2 + and creatine kinase isoenzyme.The abnormality was significantly different from the normal group (P ＜0.05 ).There were no significant differences between the p93 transgenic mice and normal group by heart mass index (HMI) measure. Conclusions The transgenic mouse model carrying p93 gene can be established.Single p93 gene does not induce AVSD and cardiac hypertrophy and may protect the heart.
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