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The research of adriamycin induced focal segmental glomerulosclerosis in rat

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WANG Chen-dan(Department of Nephrology, the Second Hospital of Shanxi Medical University,Taiyuan 030001, China)
LI Rong-shan(Department of Nephrology, the Second Hospital of Shanxi Medical University,Taiyuan 030001, China)
QIAO Xi(Department of Nephrology, the Second Hospital of Shanxi Medical University,Taiyuan 030001, China)
WANG Chen(Department of Nephrology, the Second Hospital of Shanxi Medical University,Taiyuan 030001, China)
Journal Title:
China Medicine
Volume 06, Issue 12, 2011
Key Word:
Adriamycin-induced nephropathy;Rat;Focal segmental giomeruloeclerosis

Abstract: Objective To establish the model of rat adriamycin induced nephritis (AIN)similar to the human renal glomerular disease in patho and amynology.Methods Forty Sprague Dawley rats were randomly divided into model(32) and control group (8).They were randomly divided into the first injection of adriamycin after 2 weeks,4 weeks,8 weeks and 12 weeks.Rat tail of model group vein were injected of adriamycin for two times;control group was given two injections of the same amount of normal saline through the tail veins of rats according to the method with the model group.The level of 24 hour urinary protein,serum total protein(TP),albumin(ALB),cholesterin (CH),triglyeride ( TG),serum urea nitrogen (BUN) and serum creatinine ( Cr ) were measured.The renal pathological changes were observed by light microscope and electron microscope.The expression of fibronectin(FN)and laminin (LN)was analyzed by immunohistochemistry.Matrix metalloproteinases(MMP)-2,MMP-9 and transforming grouth factor(TGF)-β1 were detected by enzyme linked immunosorbent assay.Results Compared with control group,the level of 24 hour urinary protein in AIN model at 2th,4th,8th and 12th week increased( P < 0.01 ),TP and ALB were obviously decreased[ (50.7 ± 3.6),(42.2 ± 3.4),(40.4 ± 3.1 ),(41.7 ± 3.3 ) g/L vs (62.8 ±2.8)g/L;(22.2 ±1.8),(16.4 ±1.7),(14.8 ±2.1),(13.0 ±2.2)g/L vs (29.0 ± 1.3)g/L]; TG and CH in AIN model group were obviously increased [ (5.2 ± 1.4),(6.1 ± 1.5 ),(7.3 ± 1.5 ),(7.2 ± 1.2 ) mmol/L vs (0.6 ±0.3)mmol/L;(3.8 ±1.7),(5.6 ±1.9),(8.6 ±2.7),(9.6 ±1.6)mmol/L vs (1.3 ±0.1)mmol/L] ;focal segmental glomerulosclerosis( FSGS)were observed in 12th week group; The level of TGF-β1 in serum were obviously increased,the level of MMP-2 and M MP-9 reduced [ ( 151.4 ± 2.4 ),( 100.5 ± 3.1 ),(76.5 ± 3.6),( 83.7 ±3.1 ) μg/L; (73.4 ± 2.7),(58.9 ± 2.2 ),( 35.4 ± 2.0),( 33.3 ± 1.5 ) μg/L ] ; the expression and distribution of FN,LN increased significantly.Conclusion After the first large doses of adriamycin (6 mg/kg)shockb is induced,plus one week after low dose (4 mg/kg) administration in rats,the rats appear visiblely part of giomerular appearance of FSGS at the end of 12 weeks.

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