Abstract: Objective To investigate the effect of intermedin (IMD)on neonatal rat cardiomyocytes apoptosis induced by noradrenaline (NE). Methods Totally 20 cultured neonatal rats cardiomyocytes were randomly divided into S groups:control group, NE group, IMD group,calcitonin-gene-relatedpeptide(CGRP)8-37 group and adrenal medulla(ADM)22-52 group. Cultured cardiomyocytes were given 10-6 mol/L NE. At 30 min before administration of NE, IMD was added into the culture fluid in IMD group. CGRP8-37 or ADM22-52 were added at 30 min before IMD administration in CGRP8-37 group and ADM22-52 group respectively. Cardiomyocvte apoptotic rate (AR) and Caspase-3 activity were detected. Results Compared with control group, AR and Caspase-3 activity increased in NE group[(31.95±6.33)% vs (8.70±3.92)%,(2.97±0.20)% vs (1.00±0.15)% ,P<0.05]. AR and Caspase-3 activity decreased significantly in IMD group compared with NE group(P<0.05). AR and Caspase-3 activity were higher in CGRP8-37 group and ADM22-52 group than those in IMD group (P< 0. 05). Conclusion IMD can attenuate cardiomyocyte apoptosis induced by NE through CGRP receptor and ADM receptor.