Thyroid-stimulating hormone can decreases expression of GLUT4 in 3T3-L1 adipocytes
- Author:
- No author available
- Journal Title:
- CHINA MEDICINE
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1673-4777.2011.06.017
- Key Word:
- 促甲状腺激素;脂肪细胞;葡萄糖转运蛋白质;白细胞介素6;Thyroid-stimulating hormone;Adipocyte;Glucose transporter protein;Interleukin-6
Abstract： Objective To test if thyroid-stimulating hormone(TSH) can suppress expression of glucose transporter 4(GLUT4)in 3T3-L1 adipocytes. Methods 3T3-L1 preadipocytes were induced to differentiate into adipocytes. These adipocytes were treated with 0 to 1.0 IU/L TSH for up to 48 hours. Protein levels of total GLUT4 were quantified by western blotting. Culture medium interleukin-6 level was assessed by enzyme-linked immunosor-bent assay(ELI(S)A). Results The GLUT4 protein level after 48 hours induced with different dose(0. 01 IU/L, 0.1 IU/L, 1 IU/L) of TSH presented a significant 23% , 44% , 74% ( all P < 0.05 ) reduction compared with control group. While the interleukin-6 level of culture medium increased significantly [( 101. 9300 ± 6. 8396), (104.1533 ±5.9619), (129.0167 ±6.4232) μg/L vs (93.2500 ±2.6695)μg/L, P<0.05]. Conclusion In 3T3-L1 adipocytes, TSH can decrease expression of GLUT43.
- [1]李伟,张龙江,林汉华,等.重组人白细胞介素-6对SW872脂肪细胞葡萄糖代谢和胰岛素敏感性的影响及其机制[J].2007,(8).
- [2]Antunes TT,Gagnon A,Bell A,etc.Thyroid-stimulating hormone stimulates interleukin-6 release from 3T3-L1 adipocytes through a cAMP-protein kinase A pathway.[J].2005,13(12).
- [3]Gagnon A,Langille ML,Sorisky A,etc.Thyroid-stimulating hormone induces interleukin-6 release from human adipocytes through activation of the nuclear factor-kappaB pathway.[J].2008,149(6).
- [4]Dardano A,Ghiadoni L,Plantinga Y,etc.Recombinant human thyrotropin reduces endothelium-dependent vasodilation in patients monitored for differentiated thyroid carcinoma.[J].2006,91(10).
- [5]Gagnon A,Pacini F,Smith TJ,etc.Interleukin-6 release from human abdominal adipose cells is regulated by thyroid-stimulating hormone: effect of adipocyte differentiation and anatomic depot.[J].2006,290(6).
- [6]de Lloyd A,Bursell J,Gregory J.TSH receptor activation and body composition.[J].2010,204(1).